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AAV vector analytics

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Adeno-associated viruses (AAVs) have become one of the most commonly used vectors to deliver gene therapies. As their use grows, so does the need for accurate, efficient AAV analytics. Mass photometry can monitor sample critical quality attributes (CQAs) throughout downstream workflows – accelerating manufacturing and process development, to ultimately help better therapeutics reach patients faster.

Quantification of AAV empty/full ratio

A mass photometry histogram showing how the technique can distinguish empty, full and partially filled AAV populations.

Mass photometry accurately measures empty/full AAV ratios, and resolves populations of partially filled and overfull capsids. It works by using light to measure the mass of each particle, which varies depending on the mass of the encapsidated genome.

Figure: Mass photometry resolves empty, full and partially filled AAV subpopulations. Here, populations of empty and full AAV vectors form clearly visible peaks and represent the bulk of the sample, while partially filled capsids are distributed across an intermediate mass range. The mass photometry measurement was consistent with a SEC-MALS measurement of the same sample. 

Quantification of AAV full empty ratio

Agreement with orthogonal methods

Scientists working with AAVs must choose from among a range of options in the AAV vector analytics toolbox and it may be difficult to decide whether to prioritize accuracy or usability. With mass photometry, the difficulty disappears – mass photometers are highly accurate instruments that can be used over and over, throughout AAV purification processes.

A comparison between mass photometry and AUC shows comparable estimates of the proportions of  AAV subpopulations

Mass photometry has been proven to provide comparable results to SEC-MALS, cryo-TEM and analytical ultracentrifugation (AUC) while offering important practical advantages, including speed, ease of use and suitability for use in a GMP-compliant environment.

Read more in our article, Comparing analytical approaches for AAV characterization.

Figure: Mass photometry (MP) results for AAV empty/full analysis are comparable to those obtained using AUC, a commonly used technology for AAV characterization. Source: Wagner et al., Int. J. Mol Sci, 2023.

Agreement with orthogonal methods

Transforming analytical workflows for AAV purification

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  • Results in minutes

  • Low sample requirement

  • Titer estimation

  • Benchtop instrument

  • Easy to use

  • Any serotype

  • Suitable for GMP-compliant environments

Combining speed, ease of use and low sample requirements, mass photometry integrates seamlessly into workflows for the downstream processing of AAVs in gene therapy development and manufacturing. It is also suitable for GMP-compliant environments.

Transforming analytical workflows for AAV purification

Mass photometry solutions for AAV vector analytics:
The SamuxMP product line

A mass photometer optimized for adeno-associated virus (AAV) characterization, the SamuxMP is an essential tool for AAV research, development and manufacturing. It features:

  • Characterization of AAV samples

  • Rapid analysis requiring little sample

  • User-friendly operation

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Mass photometry solutions for AAV vector analytics
What mass photometry users are saying

What mass photometry users are saying

"We're generating data to compare mass photometry with gold standards like AUC and cryoTEM. Early indications show that it’s much quicker and equally accurate. Partners who've compared mass photometry to other methods themselves vouch for its accuracy. It's a game changer in AAV characterization, for us as well as our clients."

Quentin Bazot, Process Development and Innovation Manager, ABL Europe

 

 

Read more about this user’s experience using mass photometry for AAV vector analytics

Further resources on AAV vector analytics with mass photometry

Learn more about how mass photometry can streamline your AAV purification protocols

Contact us

Refeyn and Samux are registered trademarks of Refeyn Ltd. 

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