Quantifying heterogeneous AAV loading using mass photometry
Empty/full AAV ratios and heterogenous loading in AAV analytics
Adeno-associated viruses (AAVs) are promising vectors for the delivery of recombinant DNA in gene therapies. One of the key attributes in AAV analytics is the AAV empty/full ratio, which is the proportion of viral capsids that contain the full recombinant DNA. Also of interest is the proportion of partially filled and overfilled capsids. Efficiently characterizing these attributes is vital for the development of AAV-based gene therapy products.
Mass photometry can support AAV purification protocols
Mass photometry can characterize AAV samples with accuracy comparable to gold standard methods for AAV analytics. Mass photometers like the SamuxMP and the SamuxMP Auto are tailored to provide information about critical analytical attributes of AAV gene therapy samples, providing:
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Accurate, single-particle characterization within the relevant mass range (0.5-6 MDa)
Measurement of AAV empty/full ratio, partially filled and overfilled capsids in less than 5 minutes
Requires as little as 5-10 µL of sample with a concentration of 10^11 particles/mL
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These benefits make mass photometry an ideal technique for AAV analytics. The low sample consumption of mass photometry is beneficial during the development of AAV-based gene therapy products, when AAV sample quantities are restricted and very valuable.
How to measure AAV empty/full ratios and partials with mass photometry
In this application note, you will learn how mass photometry can quantify AAV capsid loading, including AAV empty/full ratios as well as partials. You will also see how the results of mass photometry compare with those of gold standard AAV analytics methods. Download the app note and learn the full details.
Additional resources​
WEBINAR​
Harnessing mass photometry for AAV sample characterization in GMP-regulated environments
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In this webinar you can learn how mass photometry measurement of AAV samples can be applied to GMP-regulated environments such as AAV gene therapy manufacturing.
BLOG POST​
Blog: Comparing analytical approaches for AAV characterization
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Read this blog post to learn how mass photometry compares to other AAV analytical methods for measurements of empty/full and heterogeneously loaded AAV capsids.
More Application Notes
Browse through our catalogue of application notes highlighting some recent case studies featuring mass photometry.