Formation of PROTAC ternary complexes measured with mass photometry

Application Note

Small molecule-induced selective degradation of target proteins is one of the most promising new generation drug discovery approaches. Small bifunctional molecules like PROTACs can induce the recruitment of a target protein to an E3 ligase, forming a ternary complex to facilitate the target’s degradation via the cellular proteasome. This application note introduces a mass photometry-based assay, which can inform on the key aspects of the ternary complex formation. Mass photometry experiments do not require protein labelling or immobilisation and therefore allow direct visualisation and quantification of true molecule behaviour in solution.

Download the application note

Fill in your details to receive the application note via e-mail. In case you do not receive it, please check your spam or junk folder, or contact us.

By clicking submit you will be subscribed to our mailing list and will receive occasional updates from Refeyn. Your address and personal information will be stored securely. We will not sell or share this information with anyone.

 

More Application Notes

Browse through our catalogue of application notes highlighting some recent case studies featuring mass photometry.

Mass photometry of nucleic acids

Mass photometry of nanocages

Mass photometry on SARS-CoV-2

Mass photometry on AAVs

Stay connected!

Join our mailing list to receive regular updates from Refeyn.

Contact Us

Refeyn Ltd.

1 Electric Avenue, Ferry Hinksey Road,

Oxford OX20BY

  • YouTube
  • LinkedIn
  • Twitter

Useful Links