Formation of PROTAC ternary complexes measured with mass photometry

Application Note

Small molecule-induced selective degradation of target proteins is one of the most promising new generation drug discovery approaches. Small bifunctional molecules like PROTACs can induce the recruitment of a target protein to an E3 ligase, forming a ternary complex to facilitate the target’s degradation via the cellular proteasome. This application note introduces a mass photometry-based assay, which can inform on the key aspects of the ternary complex formation. Mass photometry experiments do not require protein labelling or immobilisation and therefore allow direct visualisation and quantification of true molecule behaviour in solution.

AppNote Protacs.png

More Application Notes

Browse through our catalogue of application notes highlighting some recent case studies featuring mass photometry.

Mass photometry of nucleic acids

Mass photometry of nanocages

Mass photometry on SARS-CoV-2

Mass photometry on AAVs