Featured publications

OCTOBER 2021

Cohesin mediates DNA loop extrusion by a “swing and clamp” mechanism

Bauer B.W., Davidson I.F., Canena D. et al.

Cell, 2021, ISSN 0092-8674,
https://doi.org/10.1016/j.cell.2021.09.016.

OCTOBER 2021

Weighing single protein complexes on the go

OCTOBER 2021

The characterization of protein interactions - what, how and how much?

OCTOBER 2021

Custom Design of Protein Particles as Multifunctional Biomaterials

Shanbhag, B. K., Liu, C., P. G.C., , Younas, T. et al.

Adv. Funct. Mater. 2021, 2108039. https://doi.org/10.1002/adfm.202108039

OCTOBER 2021

Mass-sensitive particle tracking to elucidate the membrane-associated MinDE reaction cycle

SEPTEMBER 2021

Assembly principles of the human R2TP chaperone complex reveal the presence of R2T and R2P complexes

AUGUST 2021

Structural features of Cryptococcus neoformans bifunctional GAR/AIR synthetase may present novel antifungal drug targets

AUGUST 2021

Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation

AUGUST 2021

Structural features of Cryptococcus neoformans bifunctional GAR/AIR synthetase may present novel antifungal drug targets

JULY 2021

Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies

Armstrong L.A., Lange S M, Cesare V D, et al. 

PLOS One (2021) https://doi.org/10.1371/journal.pone.0253364

JULY 2021

Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3

Carabias, A., Fuglsang, A., Temperini, P. et al. 

Nat Commun 12, 4476 (2021). https://doi.org/10.1038/s41467-021-24707-3

JULY 2021

Size distributions of colloidal gold nanoparticles measured in solution by single-particle mass photometry

Melo L, Hui A, Kowal M, et al. 

arXiv:2107.06247 [cond-mat.mes-hall].

JUNE 2021

Crystallographic fragment screening-based study of a novel FAD-dependent oxidoreductase from Chaetomium thermophilum 

Svecova, L., Ostergaard, L. H., Skalova, T., et al. 

J. (2021). Acta Cryst. D77, 755-775.

JUNE 2021

Probing Affinity, Avidity, Anti-Cooperativity, and Competition in Antibody and Receptor Binding to the SARS-CoV-2 Spike by Single Particle Mass Analyses

Yin V, Lai S, Caniels T G, et al.

BioRxiv doi: https://doi.org/10.1101/2021.06.18.448939

MARCH 2021

Fundamental bounds on the precision of iSCAT, COBRI and dark-field microscopy for 3D localization and mass photometry

Dong J, Dante M, Conrad-Billroth C, et al.

arXiv:2106.10758 [physics.optics]

APRIL 2021

Multiscale Modeling and Analysis for High-fidelity Interferometric Scattering Microscopy

APRIL 2021

Nanobody Repertoires for Exposing Vulnerabilities of SARS-CoV-2

MARCH 2021

Biochemical Investigation of the Interaction of pICln, RioK1 and COPR5 with the PRMT5-MEP50 Complex

Krzyzanowski A, Gasper R, Adihou H, Hart P', Waldmann H.

Chembiochem. 2021;22(11):1908-1914. doi:10.1002/cbic.202100079

MARCH 2021

Micromirror total internal reflection microscopy for high-performance single particle tracking at interfaces

Meng X, Sonn-Segev A, Schumacher A, et al. 

https://pubs.acs.org/doi/10.1021/acsphotonics.1c01268

MARCH 2021

Identification and characterization of two transmembrane proteins required for virulence of Ustilago maydis

MARCH 2021

Standard protocol for mass photometry experiments

FEBRUARY 2021

Quantifying misfolded protein oligomers as drug targets and biomarkers in Alzheimer and Parkinson diseases

Kulenkampff K, Wolf Perez AM, Sormanni P et al.

Nat Rev Chem (2021).
https://doi.org/10.1038/s41570-021-00254-9

FEBRUARY 2021

Rapid Characterization of AAV gene therapy vectors by Mass Photometry

JANUARY 2021

A binding protein regulates myosin-7a dimerization and actin bundle assembly

DECEMBER 2020

Illuminating Individual Membrane Protein Complexes with Mass Photometry

Marty M.T.

Chem, 2020, ISSN 2451-9294, https://doi.org/10.1016/j.chempr.2020.12.012.

DECEMBER 2020

Structural Characterization of Glycerol Kinase from the Thermophilic Fungus Chaetomium thermophilum

Wilk, P, Kuśka, K, Wątor, E et al.

Int. J. Mol. Sci. 2020, 21, 9570.

DECEMBER 2020

Ribosome association primes the stringent factor Rel for tRNA-dependent locking in the A-site and activation of (p)ppGpp synthesis

Takada H, Roghanian M, Caballero-Montes J, et al.

 

Nucleic Acids Research, , gkaa1187, https://doi.org/10.1093/nar/gkaa1187

DECEMBER 2020

The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity

DECEMBER 2020

High affinity modified ACE2 receptors protect from SARS-CoV-2 infection in hamsters

Higuchi Y, Suzuki T, Arimori T, et al. 

bioRxiv 2020.09.16.299891; doi: https://doi.org/10.1101/2020.09.16.299891

SEPTEMBER 2020

McsB forms a gated kinase chamber to mark aberrant bacterial proteins for degradation

AUGUST 2020

Equilibrium Binding Model for CpG DNA-Dependent Dimerization of Toll-like Receptor 9 Ectodomain

Reikine S., McLaughlin S.H., and Modis Y.

Biochemistry Article ASAP
DOI: 10.1021/acs.biochem.0c00466

AUGUST 2020

Emergence and Rearrangement of Dynamic Supramolecular Aggregates Visualized by Interferometric Scattering Microscopy

Lebedeva M.A., Palmieri E., Kukura P. et al.

ACS Nano Article ASAP. DOI: 10.1021/acsnano.0c02414

AUGUST 2020

Single molecule mass photometry of nucleic acids

Yiwen Li, Weston B. Struwe, Philipp Kukura

Nucleic Acids Research, gkaa632, https://doi.org/10.1093/nar/gkaa632

AUGUST 2020

The Phosphatase PP2A Interacts With ArnA and ArnB to Regulate the Oligomeric State and the Stability of the ArnA/B Complex

JULY 2020

Weighing single proteins with light

Strack, R.

Nat Methods 15, 477 (2018).

https://doi.org/10.1038/s41592-018-0055-6

JULY 2020

Mer2 binds directly to both nucleosomes and axial proteins as the keystone of meiotic recombination

Rousova D., K. Funk S., Reichle H., Weir J.R.

bioRxiv 2020.07.30.228908; doi: https://doi.org/10.1101/2020.07.30.228908

MAY 2020

The linear ubiquitin chain assembly complex generates heterotypic ubiquitin chains

Carvajal A.R., Diaz C.G., Vogel A., Sonn-Segev A., et al.

bioRxiv 2020.05.27.117952; doi: https://doi.org/10.1101/2020.05.27.117952

APRIL 2020

Structural basis of host protein hijacking in human T-cell leukemia virus integration

Bhatt, V., Shi, K., Salamango, D.J. et al. 

Nat Commun 11, 3121 (2020). https://doi.org/10.1038/s41467-020-16963-6

APRIL 2020

Characterization of CaMKIIα Holoenzyme Stability

APRIL 2020

Myosin II Filament Dynamics in Actin Networks Revealed with Interferometric Scattering Microscopy

MARCH 2020

Measuring the affinity of protein-protein interactions on a single-molecule level by mass photometry

APRIL 2020

Quantifying the heterogeneity of macromolecular machines by mass photometry

Sonn-Segev A, Belacic K, Bodrug T, et al. 

Nat Commun 11, 1772 (2020). https://doi.org/10.1038/s41467-020-15642-w

NOVEMBER 2019

Measurements of the self-assembly kinetics of individual viral capsids around their RNA genome

JUNE 2019

Dissecting FOXP2 Oligomerization and DNA Binding

APRIL 2019

Interferometric Scattering Microscopy

JANUARY 2017

Label-Free Single-Molecule Imaging with Numerical-Aperture-Shaped Interferometric Scattering Microscopy

OCTOBER 2021

Mass photometry enables label-free tracking and mass measurement of single proteins on lipid bilayers

Foley, E.D.B., Kushwah, M.S., Young, G. et al. 

Nat Methods 18, 1247–1252 (2021). https://doi.org/10.1038/s41592-021-01261-w

OCTOBER 2021

Single Molecule Mass Photometry Reveals the Dynamic Oligomerization of Human and Plant Peroxiredoxins

OCTOBER 2021

Single-particle mass analysis of intact ribosomes by mass photometry and Orbitrap-based charge detection mass spectrometry

OCTOBER 2021

Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease

Karvelis, T., Druteika, G., Bigelyte, G. et al. 

Nature (2021). https://doi.org/10.1038/s41586-021-04058-1

SEPTEMBER 2021

Condensin compacts DNA in 15 nm steps and requires FACT for extrusion on chromatin

Sudhakar S, Fisher G LM, Gutierrez-Escribano P et al.

bioRxiv 2021.09.15.460217; doi: https://doi.org/10.1101/2021.09.15.460217

SEPTEMBER 2021

Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins

AUGUST 2021

Linker histone H1.8 inhibits chromatin-binding of condensins and DNA topoisomerase II to tune chromosome length and individualization

Choppakatla P, Dekker B,  Cutts E E, et al.

eLife 2021;10:e68918 DOI: 10.7554/eLife.68918

AUGUST 2021

MRE11-RAD50 oligomerization promotes DNA double-strand break repair

Kissling V., Reginato G., Bianco E., et al
 

https://www.researchsquare.com/article/rs-811984/v1

AUGUST 2021

Protein cofactors and substrate influence Mg2+-dependent structural changes in the catalytic RNA of archaeal RNase P

JULY 2021

Revealing Advanced Glycation End Products Associated Structural Changes in Serum Albumin

JULY 2021

Label-free methods for optical in vitro characterization of protein–protein interaction

JUNE 2021

Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities

JUNE 2021

Biochemical consequences of two clinically relevant ND-gene mutations in Escherichia coli respiratory complex

JUNE 2021

Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2

MAY 2021

Structure and mechanistic features of the prokaryotic minimal RNase P

APRIL 2021

Distinct RPA domains promote recruitment and the helicase-nuclease activities of Dna2

Acharya A, Kasaciunaite K, Göse M, et al.

https://www.researchsquare.com/article/rs-358230/v1

MARCH 2021

Assessing Antigen Structural Integrity through Glycosylation Analysis of the SARS-CoV-2 Viral Spike

Brun J, Vasiljevic S, Gangadharan S, et al. 

https://pubs.acs.org/doi/10.1021/acscentsci.1c00058

MARCH 2021

Antibody elicited by HIV-1 immunogen vaccination in macaques displaces Env fusion peptide and destroys a neutralizing epitope

Abernathy M E, Gristick H B, Vielmetter J, et al. 

bioRxiv 2021.03.17.435265;
doi: https://doi.org/10.1101/2021.03.17.435265

MARCH 2021

Cryo-Electron Microscopy and Mass Analysis of Oligolysine-Coated DNA Nanostructures

Bertosin E, Stömmer P, Feigl E, et al. 

ACS Nano Article ASAP DOI: 10.1021/acsnano.0c10137

MARCH 2021

Reformulation of an extant ATPase active site to mimic ancestral GTPase activity reveals a nucleotide base requirement for function

Updegrove T, Harke J,  Anantharaman V,  Yang J, et al. 

eLife 2021;10:e65845 DOI:10.7554/eLife.65845

FEBRUARY 2021

Discovery of a novel lactate dehydrogenase tetramerization domain using epitope mapping and peptides

FEBRUARY 2021

Analytical Methods for Process and Product Characterization of Recombinant Adeno-Associated Virus-based Gene Therapies

FEBRUARY 2021

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

JANUARY 2021

Single Molecule Mass Photometry Reveals Dynamic Oligomerization of Plant and Human Peroxiredoxins for Functional Conservation and Diversification

Liebthal M, Kushwah MS, Kukura P, Dietz KJ

bioRxiv 2021.01.30.428949; doi: https://doi.org/10.1101/2021.01.30.428949

DECEMBER 2020

Regenerable Biosensors for Small-Molecule Kinetic Characterization Using SPR

DECEMBER 2020

Mass Photometry of Membrane Proteins

Olerinyova A, Sonn Segev A , Gault J, et al. 

Chem, 2020, ISSN 2451-9294, https://doi.org/10.1016/j.chempr.2020.11.011.

DECEMBER 2020

Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy

DECEMBER 2020

Hyper-truncated Asn355- and Asn391-glycans modulate the activity of neutrophil granule myeloperoxidase

NOVEMBER 2020

Polymerization of Bacillus subtilis MreB on a lipid membrane reveals lateral co-polymerization of MreB paralogs and strong effects of cations on filament formation

Dersch, S., Reimold, C., Stoll, J. et al. 

BMC Mol and Cell Biol 21, 76 (2020). https://doi.org/10.1186/s12860-020-00319-5

AUGUST 2020

Headless C1q: a new molecular tool to decipher its collagen‐like functions

Fouët G., Bally I., Signor L., et al.

 

FEBS Journal (August 2020), https://doi.org/10.1111/febs.1554

NOVEMBER 2020

Single-molecule microscopy for in-cell quantification of protein oligomeric stoichiometry

AUGUST 2020

Rapid high-yield expression and purification of fully post-translationally modified recombinant clusterin and mutants

Satapathy, S., Dabbs, R.A. & Wilson, M.R.

Sci Rep 10, 14243 (2020). https://doi.org/10.1038/s41598-020-70990-3

JULY 2020

Structures of fungal and plant acetohydroxyacid synthases

Lonhienne, T., Low, Y.S., Garcia, M.D. et al.

Nature (2020). https://doi.org/10.1038/s41586-020-2514-3

JULY 2020

Substrate-induced clustering activates Trim-Away of pathogens and proteins

JULY 2020

Quantifying the monomer-dimer equilibrium of tubulin with mass photometry

JULY 2020

Synthesis and Characterization of Dendrimer-Based Polysarcosine Star Polymers: Well-Defined, Versatile Platforms Designed for Drug-Delivery Applications

England R.M., Moss J.I., Gunnarsson A., et al.

Biomacromolecules 2020 21 (8), 3332-3341
DOI: 10.1021/acs.biomac.0c00768

APRIL 2020

Single-Molecule Biophysical Techniques to Study Actomyosin Force Transduction

Takagi Y., Hundt N., Fineberg A.

Coluccio L. (eds) Myosins. Advances in Experimental Medicine and Biology, vol 1239. Springer, Cham. https://doi.org/10.1007/978-3-030-38062-5_6

JUNE 2020

A wealth of genotype-specific proteoforms fine-tunes hemoglobin scavenging by haptoglobin

APRIL 2020

Quantifying protein-protein interactions by molecular counting with mass photometry

MARCH 2020

Single-molecule biosensors: Recent advances and applications

FEBRUARY 2020

Single-molecule fluorescence-based approach reveals novel mechanistic insights into small heat shock protein chaperone function

JANUARY 2020

Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas

OCTOBER 2019

Membrane insertion of soluble CLIC1 into active chloride channels is triggered by specific divalent cations

Varela L, Hendry A, Medina-Carmona E, et al.

bioRxiv 638080; doi: https://doi.org/10.1101/638080

MAY 2019

 An ultra-stable gold-coordinated protein cage displaying reversible assembly

Malay A, Miyazaki N, Biela A, et al.

Nature 2019 569:438–442 

APRIL 2018

Quantitative mass imaging of single biological macromolecules