Featured Publications
DECEMBER 2021
Biosensor-Enabled Deconvolution of the Avidity-Induced Affinity Enhancement for the SARS-CoV-2 Spike Protein and ACE2 Interaction
Gutgsell A.R., Gunnarsson A., Forssén P. et al.
Analytical Chemistry Article ASAP
DOI: 10.1021/acs.analchem.1c04372
DECEMBER 2021
Biopharmaceutical Formulations, Part 2: An Update on Analytical Techniques and Applications for Therapeutic Proteins, Viruses, Vaccines and Cells
Roesch A., Zölls S., Stadler D., et al.
DECEMBER 2021
Neuroserpin and transthyretin are extracellular chaperones that preferentially inhibit amyloid formation
DECEMBER 2021
Highly synergistic combinations of nanobodies that target SARS-CoV-2 and are resistant to escape
NOVEMBER 2021
Probing Affinity, Avidity, Anticooperativity, and Competition in Antibody and Receptor Binding to the SARS-CoV-2 Spike by Single Particle Mass Analyses
Yin V., Lai S., Caniels T.G., et al.
ACS Central Science Article ASAP
DOI: 10.1021/acscentsci.1c00804
NOVEMBER 2021
Structures of the HER2–HER3–NRG1β complex reveal a dynamic dimer interface
Diwanji, D., Trenker, R., Thaker, T.M. et al.
OCTOBER 2021
Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells
Bigelyte, G., Young, J.K., Karvelis, T. et al.
Nat Commun 12, 6191 (2021). https://doi.org/10.1038/s41467-021-26469-4
OCTOBER 2021
Cohesin mediates DNA loop extrusion by a “swing and clamp” mechanism
Bauer B.W., Davidson I.F., Canena D. et al.
Cell, 2021, ISSN 0092-8674,
https://doi.org/10.1016/j.cell.2021.09.016.
OCTOBER 2021
Weighing single protein complexes on the go
Vala, M., Piliarik, M.
Nat Methods 18, 1159–1160 (2021). https://doi.org/10.1038/s41592-021-01263-8
OCTOBER 2021
The characterization of protein interactions - what, how and how much?
Walport L.J., Low J.K.K. et al.
Chem. Soc. Rev., 2021, Advance Article. https://doi.org/10.1039/D1CS00548K
OCTOBER 2021
Custom Design of Protein Particles as Multifunctional Biomaterials
Shanbhag, B. K., Liu, C., P. G.C., , Younas, T. et al.
Adv. Funct. Mater. 2021, 2108039. https://doi.org/10.1002/adfm.202108039
OCTOBER 2021
Mass-sensitive particle tracking to elucidate the membrane-associated MinDE reaction cycle
Heermann, T., Steiert, F., Ramm, B. et al.
Nat Methods 18, 1239–1246 (2021). https://doi.org/10.1038/s41592-021-01260-x
SEPTEMBER 2021
Assembly principles of the human R2TP chaperone complex reveal the presence of R2T and R2P complexes
Seraphim T, Nano N, Cheung YW
Structure, 2021, ISSN 0969-2126, https://doi.org/10.1016/j.str.2021.08.002.
AUGUST 2021
Structural features of Cryptococcus neoformans bifunctional GAR/AIR synthetase may present novel antifungal drug targets
Chua S, Wizrah M, Luo Z, et al.
AUGUST 2021
Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation
Ranes M, Zaleska M, Sakalas S, et al.
Molecular Cell, 2021, ISSN 1097-2765, https://doi.org/10.1016/j.molcel.2021.07.013.
AUGUST 2021
Structural features of Cryptococcus neoformans bifunctional GAR/AIR synthetase may present novel antifungal drug targets
Chua S.M.H., Wizrah M.S.J., Luo Z, et al.
JULY 2021
Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies
Armstrong L.A., Lange S M, Cesare V D, et al.
PLOS One (2021) https://doi.org/10.1371/journal.pone.0253364
JULY 2021
Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3
Carabias, A., Fuglsang, A., Temperini, P. et al.
Nat Commun 12, 4476 (2021). https://doi.org/10.1038/s41467-021-24707-3
JULY 2021
Size distributions of colloidal gold nanoparticles measured in solution by single-particle mass photometry
Melo L, Hui A, Kowal M, et al.
J Phys Chem B. 2021 Nov 4. doi: 10.1021/acs.jpcb.1c05557. Epub ahead of print. PMID: 34734725.
JUNE 2021
Crystallographic fragment screening-based study of a novel FAD-dependent oxidoreductase from Chaetomium thermophilum
JUNE 2021
Probing Affinity, Avidity, Anti-Cooperativity, and Competition in Antibody and Receptor Binding to the SARS-CoV-2 Spike by Single Particle Mass Analyses
MARCH 2021
Fundamental bounds on the precision of iSCAT, COBRI and dark-field microscopy for 3D localization and mass photometry
APRIL 2021
Multiscale Modeling and Analysis for High-fidelity Interferometric Scattering Microscopy
APRIL 2021
Nanobody Repertoires for Exposing Vulnerabilities of SARS-CoV-2
Mast F D, Fridy P C, Ketaren N E, et al.
bioRxiv 2021.04.08.438911; doi: https://doi.org/10.1101/2021.04.08.438911
MARCH 2021
Biochemical Investigation of the Interaction of pICln, RioK1 and COPR5 with the PRMT5-MEP50 Complex
Krzyzanowski A, Gasper R, Adihou H, Hart P', Waldmann H.
Chembiochem. 2021;22(11):1908-1914. doi:10.1002/cbic.202100079
MARCH 2021
Micromirror total internal reflection microscopy for high-performance single particle tracking at interfaces
MARCH 2021
Identification and characterization of two transmembrane proteins required for virulence of Ustilago maydis
MARCH 2021
Standard protocol for mass photometry experiments
FEBRUARY 2021
Quantifying misfolded protein oligomers as drug targets and biomarkers in Alzheimer and Parkinson diseases
Kulenkampff K, Wolf Perez AM, Sormanni P et al.
Nat Rev Chem (2021).
https://doi.org/10.1038/s41570-021-00254-9
FEBRUARY 2021
Rapid Characterization of AAV gene therapy vectors by Mass Photometry
Wu D, Hwang P, Li T, et al.
bioRxiv 2021.02.18.431916; doi: https://doi.org/10.1101/2021.02.18.431916
JANUARY 2021
A binding protein regulates myosin-7a dimerization and actin bundle assembly
Liu R, Billington N, Yang Y et al.
Nat Commun 12, 563 (2021). https://doi.org/10.1038/s41467-020-20864-z
DECEMBER 2020
Illuminating Individual Membrane Protein Complexes with Mass Photometry
DECEMBER 2020
Structural Characterization of Glycerol Kinase from the Thermophilic Fungus Chaetomium thermophilum
DECEMBER 2020
Ribosome association primes the stringent factor Rel for tRNA-dependent locking in the A-site and activation of (p)ppGpp synthesis
Takada H, Roghanian M, Caballero-Montes J, et al.
Nucleic Acids Research, , gkaa1187, https://doi.org/10.1093/nar/gkaa1187
DECEMBER 2020
The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity
Marcaida M.J., Kauzlaric A., Duperrex A. et al.
Cell VOLUME 23, ISSUE 12, 101811, DECEMBER 18, 2020, DOI:https://doi.org/10.1016/j.isci.2020.101811
DECEMBER 2020
High affinity modified ACE2 receptors protect from SARS-CoV-2 infection in hamsters
Higuchi Y, Suzuki T, Arimori T, et al.
bioRxiv 2020.09.16.299891; doi: https://doi.org/10.1101/2020.09.16.299891
SEPTEMBER 2020
McsB forms a gated kinase chamber to mark aberrant bacterial proteins for degradation
Hajdusits B., Suskiewicz M.J., Hundt N., et al.
Elife. 2021 Jul 30;10:e63505. doi: 10.7554/eLife.63505. PMID: 34328418; PMCID: PMC8370763.
AUGUST 2020
Equilibrium Binding Model for CpG DNA-Dependent Dimerization of Toll-like Receptor 9 Ectodomain
Reikine S., McLaughlin S.H., and Modis Y.
AUGUST 2020
Emergence and Rearrangement of Dynamic Supramolecular Aggregates Visualized by Interferometric Scattering Microscopy
AUGUST 2020
Single molecule mass photometry of nucleic acids
Yiwen Li, Weston B. Struwe, Philipp Kukura
Nucleic Acids Research, gkaa632, https://doi.org/10.1093/nar/gkaa632
AUGUST 2020
The Phosphatase PP2A Interacts With ArnA and ArnB to Regulate the Oligomeric State and the Stability of the ArnA/B Complex
Ye X., Vogt M.S., van der Does C., et al.
JULY 2020
Weighing single proteins with light
JULY 2020
Mer2 binds directly to both nucleosomes and axial proteins as the keystone of meiotic recombination
Rousova D., K. Funk S., Reichle H., Weir J.R.
bioRxiv 2020.07.30.228908; doi: https://doi.org/10.1101/2020.07.30.228908
MAY 2020
The linear ubiquitin chain assembly complex generates heterotypic ubiquitin chains
Carvajal A.R., Diaz C.G., Vogel A., Sonn-Segev A., et al.
bioRxiv 2020.05.27.117952; doi: https://doi.org/10.1101/2020.05.27.117952
APRIL 2020
Structural basis of host protein hijacking in human T-cell leukemia virus integration
Bhatt, V., Shi, K., Salamango, D.J. et al.
Nat Commun 11, 3121 (2020). https://doi.org/10.1038/s41467-020-16963-6
APRIL 2020
Characterization of CaMKIIα Holoenzyme Stability
APRIL 2020
Myosin II Filament Dynamics in Actin Networks Revealed with Interferometric Scattering Microscopy
Mosby L.S., Hundt N., Young G. et al.
MARCH 2020
Measuring the affinity of protein-protein interactions on a single-molecule level by mass photometry
APRIL 2020
Quantifying the heterogeneity of macromolecular machines by mass photometry
Sonn-Segev A, Belacic K, Bodrug T, et al.
Nat Commun 11, 1772 (2020). https://doi.org/10.1038/s41467-020-15642-w
NOVEMBER 2019
Measurements of the self-assembly kinetics of individual viral capsids around their RNA genome
Garmann R F, Goldfain A M, Manoharan V N.
JUNE 2019
Dissecting FOXP2 Oligomerization and DNA Binding
Häußermann K, Young G, Kukura P, Dietz H.
Angew Chem Int Ed Engl. 2019;58(23):7662–7667. doi:10.1002/anie.201901734
APRIL 2019
Interferometric Scattering Microscopy
JANUARY 2017
Label-Free Single-Molecule Imaging with Numerical-Aperture-Shaped Interferometric Scattering Microscopy
Cole D, Young G, Weigel A, et al.
DECEMBER 2021
Comparative Analysis of Antibodies and Heavily Glycosylated Macromolecular Immune Complexes by Size-Exclusion Chromatography Multi-Angle Light Scattering, Native Charge Detection Mass Spectrometry, and Mass Photometry
den Boer MA, Lai SH, Xue X, et al.
Anal Chem. 2021 Dec 23. doi: 10.1021/acs.analchem.1c03656. Epub ahead of print. PMID: 34939405.
DECEMBER 2021
CTP-controlled liquid–liquid phase separation of ParB
Babl L., Giacomelli G., Ramm B,, et al.
DECEMBER 2021
Huntingtin structure is orchestrated by HAP40 and shows a polyglutamine expansion-specific interaction with exon 1
Harding, R.J., Deme, J.C., Hevler, J.F. et al.
Commun Biol 4, 1374 (2021). https://doi.org/10.1038/s42003-021-02895-4
DECEMBER 2021
Antiviral activity of bacterial TIR domains via immune signalling molecules
Ofir, G., Herbst, E., Baroz, M. et al.
Nature 600, 116–120 (2021). https://doi.org/10.1038/s41586-021-04098-7
NOVEMBER 2021
Purification, reconstitution, and mass analysis of archaeal RNase P, a multisubunit ribonucleoprotein enzyme
Zahurancik WJ, Norris AS, Lai SM, et al.
Methods Enzymol. 2021;659:71-103. doi: 10.1016/bs.mie.2021.07.006. Epub 2021 Sep 25. PMID: 34752299
NOVEMBER 2021
Structure of Escherichia coli cytochrome bd-II type oxidase with bound aurachin D
Grauel, A., Kägi, J., Rasmussen, T. et al.
Nat Commun 12, 6498 (2021). https://doi.org/10.1038/s41467-021-26835-2
OCTOBER 2021
Structural basis of soluble membrane attack complex packaging for clearance
Menny, A., Lukassen, M.V., Couves, E.C. et al.
Nat Commun 12, 6086 (2021). https://doi.org/10.1038/s41467-021-26366-w
OCTOBER 2021
Mass photometry enables label-free tracking and mass measurement of single proteins on lipid bilayers
Foley, E.D.B., Kushwah, M.S., Young, G. et al.
Nat Methods 18, 1247–1252 (2021). https://doi.org/10.1038/s41592-021-01261-w
OCTOBER 2021
Single Molecule Mass Photometry Reveals the Dynamic Oligomerization of Human and Plant Peroxiredoxins
Liebthal M., Kushwah M.S., Kukura P., Dietz K.
iScience, 2021, 103258, ISSN 2589-0042,
https://doi.org/10.1016/j.isci.2021.103258.
OCTOBER 2021
Single-particle mass analysis of intact ribosomes by mass photometry and Orbitrap-based charge detection mass spectrometry
Lai S, Tamara S, Heck A J R.
iScience, 2021, 103211, ISSN 2589-0042, https://doi.org/10.1016/j.isci.2021.103211.
OCTOBER 2021
Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease
Karvelis, T., Druteika, G., Bigelyte, G. et al.
SEPTEMBER 2021
Condensin compacts DNA in 15 nm steps and requires FACT for extrusion on chromatin
Sudhakar S, Fisher G LM, Gutierrez-Escribano P et al.
bioRxiv 2021.09.15.460217; doi: https://doi.org/10.1101/2021.09.15.460217
SEPTEMBER 2021
Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins
AUGUST 2021
Linker histone H1.8 inhibits chromatin-binding of condensins and DNA topoisomerase II to tune chromosome length and individualization
AUGUST 2021
MRE11-RAD50 oligomerization promotes DNA double-strand break repair
Kissling V., Reginato G., Bianco E., et al
AUGUST 2021
Protein cofactors and substrate influence Mg2+-dependent structural changes in the catalytic RNA of archaeal RNase P
Marathe I A, Lai S M, Zahurancik W J, et al.
Nucleic Acids Research, 2021;, gkab655, https://doi.org/10.1093/nar/gkab655
JULY 2021
Revealing Advanced Glycation End Products Associated Structural Changes in Serum Albumin
Naftaly A , Izgilov R, Omari E, et al.
ACS Biomaterials Science & Engineering 2021 7 (7), 3179-3189. DOI: 10.1021/acsbiomaterials.1c00387
JULY 2021
Label-free methods for optical in vitro characterization of protein–protein interaction
Soltermann F, Struwe W.B., Kukura P
Physical Chemistry Chemical Physics (IF3.676), Pub Date : 2021-08-03, DOI: 10.1039/d1cp01072g
JUNE 2021
Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities
Bolduc J, Koruza K, Luo T et al.
Redox Biology, Volume 42, 2021, 101959, ISSN 2213-2317, https://doi.org/10.1016/j.redox.2021.101959.
JUNE 2021
Biochemical consequences of two clinically relevant ND-gene mutations in Escherichia coli respiratory complex
Nuber F, Schimpf J, di Rago JP et al.
Sci Rep 11, 12641 (2021). https://doi.org/10.1038/s41598-021-91631-3
JUNE 2021
Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2
Higuchi Y, Suzuki T, Arimori T et al.
Nat Commun 12, 3802 (2021). https://doi.org/10.1038/s41467-021-24013-y
MAY 2021
Structure and mechanistic features of the prokaryotic minimal RNase P
Feyh R, Wäber N B, Prinz S, et al.
Elife. 2021 Jun 28;10:e70160. doi: 10.7554/eLife.70160. PMID: 34180399; PMCID: PMC8266387.
APRIL 2021
Distinct RPA domains promote recruitment and the helicase-nuclease activities of Dna2
MARCH 2021
Assessing Antigen Structural Integrity through Glycosylation Analysis of the SARS-CoV-2 Viral Spike
MARCH 2021
Antibody elicited by HIV-1 immunogen vaccination in macaques displaces Env fusion peptide and destroys a neutralizing epitope
Abernathy M E, Gristick H B, Vielmetter J, et al.
bioRxiv 2021.03.17.435265;
doi: https://doi.org/10.1101/2021.03.17.435265
MARCH 2021
Cryo-Electron Microscopy and Mass Analysis of Oligolysine-Coated DNA Nanostructures
MARCH 2021
Reformulation of an extant ATPase active site to mimic ancestral GTPase activity reveals a nucleotide base requirement for function
FEBRUARY 2021
Discovery of a novel lactate dehydrogenase tetramerization domain using epitope mapping and peptides
Thabault L, Liberelle M, Koruza K, et al.
FEBRUARY 2021
Analytical Methods for Process and Product Characterization of Recombinant Adeno-Associated Virus-based Gene Therapies
Gimpel AL, Katsikis G, Sha S, Maloney AJ, et al.
FEBRUARY 2021
Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
Wu D, Piszczek G.
JANUARY 2021
Single Molecule Mass Photometry Reveals Dynamic Oligomerization of Plant and Human Peroxiredoxins for Functional Conservation and Diversification
Liebthal M, Kushwah MS, Kukura P, Dietz KJ
bioRxiv 2021.01.30.428949; doi: https://doi.org/10.1101/2021.01.30.428949
DECEMBER 2020
Regenerable Biosensors for Small-Molecule Kinetic Characterization Using SPR
Gunnarsson A, Stubbs CJ, Rawlins PB, et al.
SLAS DISCOVERY: Advancing the Science of Drug Discovery. December 2020. doi:10.1177/2472555220975358
DECEMBER 2020
Mass Photometry of Membrane Proteins
Olerinyova A, Sonn Segev A , Gault J, et al.
Chem, 2020, ISSN 2451-9294, https://doi.org/10.1016/j.chempr.2020.11.011.
DECEMBER 2020
Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy
DECEMBER 2020
Hyper-truncated Asn355- and Asn391-glycans modulate the activity of neutrophil granule myeloperoxidase
Tjondro HC, Ugonotti J, Kawahara R, et al.
NOVEMBER 2020
Polymerization of Bacillus subtilis MreB on a lipid membrane reveals lateral co-polymerization of MreB paralogs and strong effects of cations on filament formation
Dersch, S., Reimold, C., Stoll, J. et al.
BMC Mol and Cell Biol 21, 76 (2020). https://doi.org/10.1186/s12860-020-00319-5
AUGUST 2020
Headless C1q: a new molecular tool to decipher its collagen‐like functions
NOVEMBER 2020
Single-molecule microscopy for in-cell quantification of protein oligomeric stoichiometry
C. Huanhuan, X. Xihong, C. Tai-Yen
AUGUST 2020
Rapid high-yield expression and purification of fully post-translationally modified recombinant clusterin and mutants
Satapathy, S., Dabbs, R.A. & Wilson, M.R.
Sci Rep 10, 14243 (2020). https://doi.org/10.1038/s41598-020-70990-3
JULY 2020
Structures of fungal and plant acetohydroxyacid synthases
Lonhienne, T., Low, Y.S., Garcia, M.D. et al.
JULY 2020
Substrate-induced clustering activates Trim-Away of pathogens and proteins
Zeng J., Santos A.F., Mukadam A., et. al.
bioRxiv 2020.07.28.225359; doi: https://doi.org/10.1101/2020.07.28.225359
JULY 2020
Quantifying the monomer-dimer equilibrium of tubulin with mass photometry
Fineberg A., Surrey T., Kukura P.
JULY 2020
Synthesis and Characterization of Dendrimer-Based Polysarcosine Star Polymers: Well-Defined, Versatile Platforms Designed for Drug-Delivery Applications
England R.M., Moss J.I., Gunnarsson A., et al.
Biomacromolecules 2020 21 (8), 3332-3341
DOI: 10.1021/acs.biomac.0c00768
APRIL 2020
Single-Molecule Biophysical Techniques to Study Actomyosin Force Transduction
Takagi Y., Hundt N., Fineberg A.
Coluccio L. (eds) Myosins. Advances in Experimental Medicine and Biology, vol 1239. Springer, Cham. https://doi.org/10.1007/978-3-030-38062-5_6
JUNE 2020
A wealth of genotype-specific proteoforms fine-tunes hemoglobin scavenging by haptoglobin
Tamara S, Franc V, Heck A.J.R.
Proceedings of the National Academy of Sciences Jun 2020, 202002483; DOI: 10.1073/pnas.2002483117
APRIL 2020
Quantifying protein-protein interactions by molecular counting with mass photometry
Soltermann F, Foley E, Pagnoni V, et al.
Angew. Chem. Int. Ed. 2020, 59,1–6. https://doi.org/10.1002/anie.202001578
MARCH 2020
Single-molecule biosensors: Recent advances and applications
Akkilic N., Geschwindner S., Höök F.
FEBRUARY 2020
Single-molecule fluorescence-based approach reveals novel mechanistic insights into small heat shock protein chaperone function
Johnston C, Marzano N , Paudel B, et al.
bioRxiv 2020.02.16.951632; doi: https://doi.org/10.1101/2020.02.16.951632
JANUARY 2020
Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas
Hickman A, Kailasan S, Genzor P, et al.
OCTOBER 2019
Membrane insertion of soluble CLIC1 into active chloride channels is triggered by specific divalent cations
MAY 2019
An ultra-stable gold-coordinated protein cage displaying reversible assembly
APRIL 2018