Featured Publications


Biosensor-Enabled Deconvolution of the Avidity-Induced Affinity Enhancement for the SARS-CoV-2 Spike Protein and ACE2 Interaction

Gutgsell A.R., Gunnarsson A., Forssén P. et al.

Analytical Chemistry Article ASAP
DOI: 10.1021/acs.analchem.1c04372


Biopharmaceutical Formulations, Part 2: An Update on Analytical Techniques and Applications for Therapeutic Proteins, Viruses, Vaccines and Cells


Neuroserpin and transthyretin are extracellular chaperones that preferentially inhibit amyloid formation

West, J., Satapathy, S., Whiten, D.R., et al. 

Science Advances, 7(50), 2021. p.eabf7606.


Highly synergistic combinations of nanobodies that target SARS-CoV-2 and are resistant to escape

Mast F D, Fridy P C, Ketaren N E, et al. 

eLife 2021;10:e73027 DOI: 10.7554/eLife.73027.


Probing Affinity, Avidity, Anticooperativity, and Competition in Antibody and Receptor Binding to the SARS-CoV-2 Spike by Single Particle Mass Analyses


Structures of the HER2–HER3–NRG1β complex reveal a dynamic dimer interface

Diwanji, D., Trenker, R., Thaker, T.M. et al.

Nature (2021). https://doi.org/10.1038/s41586-021-04084-z


Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells

Bigelyte, G., Young, J.K., Karvelis, T. et al.

Nat Commun 12, 6191 (2021). https://doi.org/10.1038/s41467-021-26469-4


Cohesin mediates DNA loop extrusion by a “swing and clamp” mechanism

Bauer B.W., Davidson I.F., Canena D. et al.

Cell, 2021, ISSN 0092-8674,


Weighing single protein complexes on the go


The characterization of protein interactions - what, how and how much?


Custom Design of Protein Particles as Multifunctional Biomaterials

Shanbhag, B. K., Liu, C., P. G.C., , Younas, T. et al.

Adv. Funct. Mater. 2021, 2108039. https://doi.org/10.1002/adfm.202108039


Mass-sensitive particle tracking to elucidate the membrane-associated MinDE reaction cycle


Assembly principles of the human R2TP chaperone complex reveal the presence of R2T and R2P complexes


Structural features of Cryptococcus neoformans bifunctional GAR/AIR synthetase may present novel antifungal drug targets


Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation


Structural features of Cryptococcus neoformans bifunctional GAR/AIR synthetase may present novel antifungal drug targets

JULY 2021

Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies

Armstrong L.A., Lange S M, Cesare V D, et al. 

PLOS One (2021) https://doi.org/10.1371/journal.pone.0253364

JULY 2021

Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3

Carabias, A., Fuglsang, A., Temperini, P. et al. 

Nat Commun 12, 4476 (2021). https://doi.org/10.1038/s41467-021-24707-3

JULY 2021

Size distributions of colloidal gold nanoparticles measured in solution by single-particle mass photometry

JUNE 2021

Crystallographic fragment screening-based study of a novel FAD-dependent oxidoreductase from Chaetomium thermophilum 

Svecova, L., Ostergaard, L. H., Skalova, T., et al. 

J. (2021). Acta Cryst. D77, 755-775.

JUNE 2021

Probing Affinity, Avidity, Anti-Cooperativity, and Competition in Antibody and Receptor Binding to the SARS-CoV-2 Spike by Single Particle Mass Analyses

Yin V, Lai S, Caniels T G, et al.

BioRxiv doi: https://doi.org/10.1101/2021.06.18.448939

MARCH 2021

Fundamental bounds on the precision of iSCAT, COBRI and dark-field microscopy for 3D localization and mass photometry

Dong J, Dante M, Conrad-Billroth C, et al.

arXiv:2106.10758 [physics.optics]

APRIL 2021

Multiscale Modeling and Analysis for High-fidelity Interferometric Scattering Microscopy

APRIL 2021

Nanobody Repertoires for Exposing Vulnerabilities of SARS-CoV-2

MARCH 2021

Biochemical Investigation of the Interaction of pICln, RioK1 and COPR5 with the PRMT5-MEP50 Complex

Krzyzanowski A, Gasper R, Adihou H, Hart P', Waldmann H.

Chembiochem. 2021;22(11):1908-1914. doi:10.1002/cbic.202100079

MARCH 2021

Micromirror total internal reflection microscopy for high-performance single particle tracking at interfaces

Meng X, Sonn-Segev A, Schumacher A, et al. 


MARCH 2021

Identification and characterization of two transmembrane proteins required for virulence of Ustilago maydis

MARCH 2021

Standard protocol for mass photometry experiments


Quantifying misfolded protein oligomers as drug targets and biomarkers in Alzheimer and Parkinson diseases

Kulenkampff K, Wolf Perez AM, Sormanni P et al.

Nat Rev Chem (2021).


Rapid Characterization of AAV gene therapy vectors by Mass Photometry


A binding protein regulates myosin-7a dimerization and actin bundle assembly


Illuminating Individual Membrane Protein Complexes with Mass Photometry

Marty M.T.

Chem, 2020, ISSN 2451-9294, https://doi.org/10.1016/j.chempr.2020.12.012.


Structural Characterization of Glycerol Kinase from the Thermophilic Fungus Chaetomium thermophilum

Wilk, P, Kuśka, K, Wątor, E et al.

Int. J. Mol. Sci. 2020, 21, 9570.


Ribosome association primes the stringent factor Rel for tRNA-dependent locking in the A-site and activation of (p)ppGpp synthesis

Takada H, Roghanian M, Caballero-Montes J, et al.


Nucleic Acids Research, , gkaa1187, https://doi.org/10.1093/nar/gkaa1187


The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity


High affinity modified ACE2 receptors protect from SARS-CoV-2 infection in hamsters

Higuchi Y, Suzuki T, Arimori T, et al. 

bioRxiv 2020.09.16.299891; doi: https://doi.org/10.1101/2020.09.16.299891


McsB forms a gated kinase chamber to mark aberrant bacterial proteins for degradation


Equilibrium Binding Model for CpG DNA-Dependent Dimerization of Toll-like Receptor 9 Ectodomain

Reikine S., McLaughlin S.H., and Modis Y.

Biochemistry Article ASAP
DOI: 10.1021/acs.biochem.0c00466


Emergence and Rearrangement of Dynamic Supramolecular Aggregates Visualized by Interferometric Scattering Microscopy

Lebedeva M.A., Palmieri E., Kukura P. et al.

ACS Nano Article ASAP. DOI: 10.1021/acsnano.0c02414


Single molecule mass photometry of nucleic acids

Yiwen Li, Weston B. Struwe, Philipp Kukura

Nucleic Acids Research, gkaa632, https://doi.org/10.1093/nar/gkaa632


The Phosphatase PP2A Interacts With ArnA and ArnB to Regulate the Oligomeric State and the Stability of the ArnA/B Complex

JULY 2020

Weighing single proteins with light

Strack, R.

Nat Methods 15, 477 (2018).


JULY 2020

Mer2 binds directly to both nucleosomes and axial proteins as the keystone of meiotic recombination

Rousova D., K. Funk S., Reichle H., Weir J.R.

bioRxiv 2020.07.30.228908; doi: https://doi.org/10.1101/2020.07.30.228908

MAY 2020

The linear ubiquitin chain assembly complex generates heterotypic ubiquitin chains

Carvajal A.R., Diaz C.G., Vogel A., Sonn-Segev A., et al.

bioRxiv 2020.05.27.117952; doi: https://doi.org/10.1101/2020.05.27.117952

APRIL 2020

Structural basis of host protein hijacking in human T-cell leukemia virus integration

Bhatt, V., Shi, K., Salamango, D.J. et al. 

Nat Commun 11, 3121 (2020). https://doi.org/10.1038/s41467-020-16963-6

APRIL 2020

Characterization of CaMKIIα Holoenzyme Stability

APRIL 2020

Myosin II Filament Dynamics in Actin Networks Revealed with Interferometric Scattering Microscopy

MARCH 2020

Measuring the affinity of protein-protein interactions on a single-molecule level by mass photometry

APRIL 2020

Quantifying the heterogeneity of macromolecular machines by mass photometry

Sonn-Segev A, Belacic K, Bodrug T, et al. 

Nat Commun 11, 1772 (2020). https://doi.org/10.1038/s41467-020-15642-w


Measurements of the self-assembly kinetics of individual viral capsids around their RNA genome

JUNE 2019

Dissecting FOXP2 Oligomerization and DNA Binding

APRIL 2019

Interferometric Scattering Microscopy


Label-Free Single-Molecule Imaging with Numerical-Aperture-Shaped Interferometric Scattering Microscopy


Comparative Analysis of Antibodies and Heavily Glycosylated Macromolecular Immune Complexes by Size-Exclusion Chromatography Multi-Angle Light Scattering, Native Charge Detection Mass Spectrometry, and Mass Photometry


CTP-controlled liquid–liquid phase separation of ParB


Huntingtin structure is orchestrated by HAP40 and shows a polyglutamine expansion-specific interaction with exon 1

Harding, R.J., Deme, J.C., Hevler, J.F. et al. 

Commun Biol 4, 1374 (2021). https://doi.org/10.1038/s42003-021-02895-4


Antiviral activity of bacterial TIR domains via immune signalling molecules


Purification, reconstitution, and mass analysis of archaeal RNase P, a multisubunit ribonucleoprotein enzyme


Structure of Escherichia coli cytochrome bd-II type oxidase with bound aurachin D

Grauel, A., Kägi, J., Rasmussen, T. et al.

Nat Commun 12, 6498 (2021). https://doi.org/10.1038/s41467-021-26835-2


Structural basis of soluble membrane attack complex packaging for clearance

Menny, A., Lukassen, M.V., Couves, E.C. et al.

Nat Commun 12, 6086 (2021). https://doi.org/10.1038/s41467-021-26366-w


Mass photometry enables label-free tracking and mass measurement of single proteins on lipid bilayers

Foley, E.D.B., Kushwah, M.S., Young, G. et al. 

Nat Methods 18, 1247–1252 (2021). https://doi.org/10.1038/s41592-021-01261-w


Single Molecule Mass Photometry Reveals the Dynamic Oligomerization of Human and Plant Peroxiredoxins


Single-particle mass analysis of intact ribosomes by mass photometry and Orbitrap-based charge detection mass spectrometry


Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease

Karvelis, T., Druteika, G., Bigelyte, G. et al. 

Nature (2021). https://doi.org/10.1038/s41586-021-04058-1


Condensin compacts DNA in 15 nm steps and requires FACT for extrusion on chromatin

Sudhakar S, Fisher G LM, Gutierrez-Escribano P et al.

bioRxiv 2021.09.15.460217; doi: https://doi.org/10.1101/2021.09.15.460217


Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins


Linker histone H1.8 inhibits chromatin-binding of condensins and DNA topoisomerase II to tune chromosome length and individualization

Choppakatla P, Dekker B,  Cutts E E, et al.

eLife 2021;10:e68918 DOI: 10.7554/eLife.68918


MRE11-RAD50 oligomerization promotes DNA double-strand break repair

Kissling V., Reginato G., Bianco E., et al



Protein cofactors and substrate influence Mg2+-dependent structural changes in the catalytic RNA of archaeal RNase P

JULY 2021

Revealing Advanced Glycation End Products Associated Structural Changes in Serum Albumin

JULY 2021

Label-free methods for optical in vitro characterization of protein–protein interaction

JUNE 2021

Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities

JUNE 2021

Biochemical consequences of two clinically relevant ND-gene mutations in Escherichia coli respiratory complex

JUNE 2021

Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2

MAY 2021

Structure and mechanistic features of the prokaryotic minimal RNase P

APRIL 2021

Distinct RPA domains promote recruitment and the helicase-nuclease activities of Dna2

Acharya A, Kasaciunaite K, Göse M, et al.


MARCH 2021

Assessing Antigen Structural Integrity through Glycosylation Analysis of the SARS-CoV-2 Viral Spike

Brun J, Vasiljevic S, Gangadharan S, et al. 


MARCH 2021

Antibody elicited by HIV-1 immunogen vaccination in macaques displaces Env fusion peptide and destroys a neutralizing epitope

Abernathy M E, Gristick H B, Vielmetter J, et al. 

bioRxiv 2021.03.17.435265;
doi: https://doi.org/10.1101/2021.03.17.435265

MARCH 2021

Cryo-Electron Microscopy and Mass Analysis of Oligolysine-Coated DNA Nanostructures

Bertosin E, Stömmer P, Feigl E, et al. 

ACS Nano Article ASAP DOI: 10.1021/acsnano.0c10137

MARCH 2021

Reformulation of an extant ATPase active site to mimic ancestral GTPase activity reveals a nucleotide base requirement for function

Updegrove T, Harke J,  Anantharaman V,  Yang J, et al. 

eLife 2021;10:e65845 DOI:10.7554/eLife.65845


Discovery of a novel lactate dehydrogenase tetramerization domain using epitope mapping and peptides


Analytical Methods for Process and Product Characterization of Recombinant Adeno-Associated Virus-based Gene Therapies


Rapid Determination of Antibody-Antigen Affinity by Mass Photometry


Single Molecule Mass Photometry Reveals Dynamic Oligomerization of Plant and Human Peroxiredoxins for Functional Conservation and Diversification

Liebthal M, Kushwah MS, Kukura P, Dietz KJ

bioRxiv 2021.01.30.428949; doi: https://doi.org/10.1101/2021.01.30.428949


Regenerable Biosensors for Small-Molecule Kinetic Characterization Using SPR


Mass Photometry of Membrane Proteins

Olerinyova A, Sonn Segev A , Gault J, et al. 

Chem, 2020, ISSN 2451-9294, https://doi.org/10.1016/j.chempr.2020.11.011.


Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy


Hyper-truncated Asn355- and Asn391-glycans modulate the activity of neutrophil granule myeloperoxidase


Polymerization of Bacillus subtilis MreB on a lipid membrane reveals lateral co-polymerization of MreB paralogs and strong effects of cations on filament formation

Dersch, S., Reimold, C., Stoll, J. et al. 

BMC Mol and Cell Biol 21, 76 (2020). https://doi.org/10.1186/s12860-020-00319-5


Headless C1q: a new molecular tool to decipher its collagen‐like functions

Fouët G., Bally I., Signor L., et al.


FEBS Journal (August 2020), https://doi.org/10.1111/febs.1554


Single-molecule microscopy for in-cell quantification of protein oligomeric stoichiometry


Rapid high-yield expression and purification of fully post-translationally modified recombinant clusterin and mutants

Satapathy, S., Dabbs, R.A. & Wilson, M.R.

Sci Rep 10, 14243 (2020). https://doi.org/10.1038/s41598-020-70990-3

JULY 2020

Structures of fungal and plant acetohydroxyacid synthases

Lonhienne, T., Low, Y.S., Garcia, M.D. et al.

Nature (2020). https://doi.org/10.1038/s41586-020-2514-3

JULY 2020

Substrate-induced clustering activates Trim-Away of pathogens and proteins

JULY 2020

Quantifying the monomer-dimer equilibrium of tubulin with mass photometry

JULY 2020

Synthesis and Characterization of Dendrimer-Based Polysarcosine Star Polymers: Well-Defined, Versatile Platforms Designed for Drug-Delivery Applications

England R.M., Moss J.I., Gunnarsson A., et al.

Biomacromolecules 2020 21 (8), 3332-3341
DOI: 10.1021/acs.biomac.0c00768

APRIL 2020

Single-Molecule Biophysical Techniques to Study Actomyosin Force Transduction

Takagi Y., Hundt N., Fineberg A.

Coluccio L. (eds) Myosins. Advances in Experimental Medicine and Biology, vol 1239. Springer, Cham. https://doi.org/10.1007/978-3-030-38062-5_6

JUNE 2020

A wealth of genotype-specific proteoforms fine-tunes hemoglobin scavenging by haptoglobin

APRIL 2020

Quantifying protein-protein interactions by molecular counting with mass photometry

MARCH 2020

Single-molecule biosensors: Recent advances and applications


Single-molecule fluorescence-based approach reveals novel mechanistic insights into small heat shock protein chaperone function


Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas


Membrane insertion of soluble CLIC1 into active chloride channels is triggered by specific divalent cations

Varela L, Hendry A, Medina-Carmona E, et al.

bioRxiv 638080; doi: https://doi.org/10.1101/638080

MAY 2019

 An ultra-stable gold-coordinated protein cage displaying reversible assembly

Malay A, Miyazaki N, Biela A, et al.

Nature 2019 569:438–442 

APRIL 2018

Quantitative mass imaging of single biological macromolecules