Mass photometry of nanocages

Application Note

Ferritins naturally form nanocages to store and transport iron and are of interest in the fields of nanomedicine and drug delivery. The interior cavity of ferritins can be loaded with a variety of small molecules upon pH-induced disassembly and reassembly and the outer surface functionalised for targeting or soliciting a host response.

 

Here, we show how mass photometry can be used to quantify assembly, load and homogeneity at single particle resolution. Such measurements use little sample, are fast, label-free, and broadly applicable to natural and synthentic nanocages.