In this webinar, Dr. Eduard Ebberink, a postdoc in Albert Heck’s group at Ultrecht University, describes how he used mass photometry and CDMS to assess AAV capsid loading, and compares the results from the two single-particle techniques. He studied different AAV serotypes – with empty and full capsids, and generated using different production platforms (human vs. insect) – and clarifies the advantages of using such methods for this type of analysis.

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In this webinar, Santoshkumar Khatwani (Director of Analytical Development at Sangamo Therapeutics) presents his work on establishing an analytical workflow for gene therapy products followed by James Wilkinson (VP of Corporate Development at Refeyn), who gives an overview of how mass photometry works and introduces new developments in the SamuxMP product line. These include an automation upgrade and upcoming GMP-compliant software, which will render mass photometry the ideal solution for production and QC environments.

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Have you ever wondered how the CO2-fixing enzyme Rubisco Form I became the most prevalent enzyme on Earth? In  this webinar, Luca Schulz from the Max Planck Institute for Terrestrial Microbiology, used ancestral sequence reconstruction and mass photometry to determine when during its evolution, ancestral Rubisco gained structural complexity by interacting with a novel subunit. This structural change led to the increased CO2 specificity and carboxylation efficiency of modern Rubiscos.

In this webinar, Svea Cheeseman, Director of Product Management for Cell and Gene Therapy at Refeyn, discusses how mass photometry can be used to characterize AAV capsids – by measuring the empty/full capsid ratio for AAVs of any serotype with low sample consumption. She also introduces the SamuxMP Auto, Refeyn’s new automated mass photometer. The instrument streamlines the AAV characterization process and enables more efficient process development for AAV-based therapeutics.

La photométrie de masse, est une technique bio-analytique dédiée à la caractérisation des molécules. La technologie mesure la masse moléculaire des molécules entre 30 kDa à 5MDa, dans leur états natifs, en solution pour laquelle chaque molécule est détectée et quantifiée. Durant la présentation, on vous explique le concept de notre technologie et comment elle peut être appliquée dans plusieurs travaux de recherche, y compris, la quantification stœchiométrique des interactions, la caractérisation des protéines, la formation des complexes, le contrôle qualité et encore plus.

In this webinar, our product manager Svea Cheeseman provides insight into a novel mass photometry instrument tailored to address the challenges of AAV characterization. Moreover, we will see data proving its ability to reliably quantify the empty-full particle ratio for different AAV serotypes and at different purification levels, including benchmark data comparing the mass photometry solution with cryoTEM and analytical ultra-centrifugation.

In this webinar, Dr Nikolas Hundt (Ludwigs-Maximilian-University Munich), describes a new mass photometry strategy for unlabelled molecules diffusing on supported lipid bilayers. With this approach, called mass-sensitive particle tracking (MSPT), researchers can determine the mass distributions and diffusion characteristics of membrane-associated protein complexes and observe protein assembly dynamics on a lipid interface in real time.

Dr Grzegorz Piszczek (Director of the Biophysics Core Facility, NIH) and Fabian Soltermann (PhD candidate, Oxford University) discuss how mass photometry can be used to characterise and determine the purity, stoichiometry, binding affinity and kinetics of multi-protein interactions. They also cover a recent application of mass photometry: quantifying interactions of antibodies with Fc-receptors.

This webinar features our co-founder, Prof. Philipp Kukura, describing how mass photometry offers a universal way to study biomolecules, as well as its strengths and limitations.

In this webinar, Dr. Nikolas Hundt, a researcher at Ludwig Maximilian University of Munich, describes how he used mass photometry to investigate actin filament formation – by quickly quantifying all the species present in solution throughout the polymerization reaction. Mass photometry led him to a new interpretation of the mechanism behind actin polymerization and helped him revise established kinetic models.

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Miniature CRISPR-Cas ribonucleoprotein complexes are of huge interest for genome editing applications due to their simplified cellular delivery. In this webinar, Selgar Henkel-Heinecke from Leipzig University describes how he and his colleagues used mass photometry to measure the stoichiometry of two miniature Cas12f1 ribonucleoprotein complexes, AsCas12f1 and SpCas12f1 in vitro, as well as their interaction with nucleic acids that are involved in the genome editing process.

The speakers will present work studying two examples of macromolecular complexes formed by eukaryotic membrane proteins. In both cases, mass photometry was crucial to addressing highly relevant physiological questions. They will explain what mass photometry can do, and what makes it suitable for studies of membrane protein complexes.

In this webinar, find an explanation on how the macrocyclic peptides can be used as a novel toolbox to study retromer-mediated endosomal trafficking and for therapeutic targeting of retromer function. Highlighted, is the method mass photometry, which enables characterization of protein complexes.

In this webinar, we discuss in detail, automated mass photometry and show data on some of its most attractive applications—such as screening and titration assays. We illustrate how automation and the associated improvement in reproducibility can make biomolecular characterization with mass photometry even easier (TwoMP Auto Launch Webinar)

Characterizing the mass of proteins and their complexes has a wide range of applications. However, the large degree of heterogeneity present in some biological systems has historically posed a near-unsurmountable challenge to traditional native mass spectrometry and classic structural biology techniques. To overcome this, researchers are turning to new, single-particle mass analysis methods. In this webinar, Victor Yin, a postdoctoral researcher of biomolecular mass spectrometry and proteomics at Utrecht University, discusses how mass photometry and charge detection mass spectrometry enable the study of several challenging protein systems, including the interaction of full antibodies with SARS-CoV-2.

Prof. Margaret Stratton (UMass Amherst) presents her work on the role of CaMKII​ in different tissues. Mass photometry helps to quantitatively determine the stoichiometry of different variants of this crucial oligomeric enzyme.

In this webinar, Dr Adar Sonn-Segev presents how mass photometry provides quantitative information on sample heterogeneity using minimal volumes with molecular resolution. She also discusses how this approach applies to several different workflows including chemical crosslinking and multi-step purification, comparing it to the standard tools for measuring sample purity.

In this webinar, Refeyn co-founder Prof. Justin Benesch discusses examples of mass photometry measurements for large protein complexes and draws comparisons with mass measurements obtained from native mass spectrometry and other techniques. Dr Nikolas Hundt also describes the use of mass photometry to visualise the nucleation process of actin filaments in real time.