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Mass Photometry Webinars

Mass Photometry Webinars

We have hosted a range of webinars, where we discuss applications of mass photometry as well as how to get the best out of our technology. You can watch any of them on this page. To stay updated on upcoming webinars, please check out our events calendar.

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Studying Lipoprotein Lipase oligomerization dynamics with cryoEM and mass photometry

In this webinar, you will learn how Dr. Kathryn Gunn and her colleagues from University of North Carolina at Chapel Hill, solved the structure of an active lipoprotein lipase (LPL) dimer and determined its oligomerization behaviours using cutting edge bioanalytical methods. You will discover how mass photometry can complement cryoEM data – determining the dynamic oligomerization states of LPL in solution, at varying concentrations and in the presence of additives.

An essential PAT technology for downstream processing in AAV manufacturing

In this webinar, discover the role mass photometry can play in characterizing AAV samples. Through a case study presented by ABL, a CDMO providing end-to-end viral vector manufacturing services, you will learn how mass photometry expedites early process development, helps optimize the polishing step, and rapidly determines critical quality attributes of AAV samples. You can also gain insights into the broader applications of mass photometry and learn about a tailored software solution for GMP-regulated environments.

Advances in cell and gene therapy: Characterizing large viral vectors using macro mass photometry

In this webinar, Refeyn’s Laura Pala will present case studies and benchmark results on how the KaritroMP employs macro mass photometry to facilitate fast, straightforward, and qualitative analysis of single viral particles in cell and gene therapy applications. She will also show that it is well-suited for comparing different production and purification methods, as well as assessing batch-to-batch variations – ensuring the safety and efficacy of pharmaceutical products brought to market.

Streamlining AAV Characterization with Mass Photometry

In this webinar, Gael Nicolas explains how mass photometry can quickly and accurately measure the empty/full AAV capsid ratio in solution and how it overcomes the barriers of large capital expenses and the need for highly skilled operators. This makes mass photometry a practical choice for integration throughout the AAV development and manufacturing processes. Finally, Gael talks about new software solutions that enable the use of SamuxMP mass photometers in GMP-regulated environments.

Architecture and Stoichiometry of Human Shelterin Using Single-Particle EM and Mass Analysis

In this webinar, you will learn how Dr. John Zinder and his colleagues from Rockefeller University, reconstituted the telomere protector, shelterin, and how they structurally characterized it with cutting-edge bioanalytical methods. You will discover how mass photometry can be used orthogonally to negative stain EM, mass spectrometry and structural modeling – determining the composition and structural heterogeneity of shelterin subcomplexes. Ultimately, you will learn how revealing the complex’s flexibility can pave the way for further investigations into potential therapeutic strategies targeting telomere-related diseases.

Assessing production variability in adeno-associated viruses by single-particle mass analysis

In this webinar, Dr. Eduard Ebberink, a postdoc in Albert Heck’s group at Ultrecht University, describes how he used mass photometry and CDMS to assess AAV capsid loading, and compares the results from the two single-particle techniques. He studied different AAV serotypes – with empty and full capsids, and generated using different production platforms (human vs. insect) – and clarifies the advantages of using such methods for this type of analysis.

Demonstrating Analytical Comparability in Analytical Development

In this webinar, Santoshkumar Khatwani (Director of Analytical Development at Sangamo Therapeutics) presents his work on establishing an analytical workflow for gene therapy products followed by James Wilkinson (VP of Corporate Development at Refeyn), who gives an overview of how mass photometry works and introduces new developments in the SamuxMP product line. These include an automation upgrade and upcoming GMP-compliant software, which will render mass photometry the ideal solution for production and QC environments.

The evolution of carbon fixation: Studying Form I Rubisco using ancestral sequence reconstruction and mass photometry

Have you ever wondered how the CO2-fixing enzyme Rubisco Form I became the most prevalent enzyme on Earth? In  this webinar, Luca Schulz from the Max Planck Institute for Terrestrial Microbiology, used ancestral sequence reconstruction and mass photometry to determine when during its evolution, ancestral Rubisco gained structural complexity by interacting with a novel subunit. This structural change led to the increased CO2 specificity and carboxylation efficiency of modern Rubiscos.

Streamlining AAV characterization with automated mass photometry

In this webinar, Svea Cheeseman, Director of Product Management for Cell and Gene Therapy at Refeyn, discusses how mass photometry can be used to characterize AAV capsids – by measuring the empty/full capsid ratio for AAVs of any serotype with low sample consumption. She also introduces the SamuxMP Auto, Refeyn’s new automated mass photometer. The instrument streamlines the AAV characterization process and enables more efficient process development for AAV-based therapeutics.

La photométrie de masse: Caractériser vos molécules en 5 minutes!

La photométrie de masse, est une technique bio-analytique dédiée à la caractérisation des molécules. La technologie mesure la masse moléculaire des molécules entre 30 kDa à 5MDa, dans leur états natifs, en solution pour laquelle chaque molécule est détectée et quantifiée. Durant la présentation, on vous explique le concept de notre technologie et comment elle peut être appliquée dans plusieurs travaux de recherche, y compris, la quantification stœchiométrique des interactions, la caractérisation des protéines, la formation des complexes, le contrôle qualité et encore plus.

Are Your AAVs Filled? Determine Rapidly with Mass Photometry

In this webinar, our product manager Svea Cheeseman provides insight into a novel mass photometry instrument tailored to address the challenges of AAV characterization. Moreover, we will see data proving its ability to reliably quantify the empty-full particle ratio for different AAV serotypes and at different purification levels, including benchmark data comparing the mass photometry solution with cryoTEM and analytical ultra-centrifugation.

Measuring Membrane Proteins with Mass-Sensitive Particle Tracking

In this webinar, Dr Nikolas Hundt (Ludwigs-Maximilian-University Munich), describes a new mass photometry strategy for unlabelled molecules diffusing on supported lipid bilayers. With this approach, called mass-sensitive particle tracking (MSPT), researchers can determine the mass distributions and diffusion characteristics of membrane-associated protein complexes and observe protein assembly dynamics on a lipid interface in real time.

Quantifying protein-protein interactions by molecular counting with mass photometry

Dr Grzegorz Piszczek (Director of the Biophysics Core Facility, NIH) and Fabian Soltermann (PhD candidate, Oxford University) discuss how mass photometry can be used to characterise and determine the purity, stoichiometry, binding affinity and kinetics of multi-protein interactions. They also cover a recent application of mass photometry: quantifying interactions of antibodies with Fc-receptors.

Measuring Molecules with Light

This webinar features our co-founder, Prof. Philipp Kukura, describing how mass photometry offers a universal way to study biomolecules, as well as its strengths and limitations.

Therapeutic antibody stability – a rapid method for forced antibody degradation studies

In this webinar forced antibody degradation studies are essential for evaluating susceptibility to aggregation or fragmentation, critical in biotherapeutic development. In these studies, analytical tools play a pivotal role, ensuring precise characterization of samples exposed to different conditions. In this webinar, you can learn how mass photometry, a column-free technology, can quickly assess antibody sample quality, accommodating diverse buffers and requiring minimal sample amount. Through case studies, you can explore the utility of mass photometry in characterizing antibody aggregation, and see how it compares to the gold-standard size-exclusion chromatography.

Tau protein aggregation & neurodegenerative disease; new insights from mass photometry

In this webinar, you will learn how Dr. Simanta Sarani Paul and his colleagues from the University of Alberta used mass photometry, a single-molecule, label-free technology, to overcome these limitations. Simanta will demonstrate how mass photometry can precisely measure tau protein oligomers in solution, capturing their different sizes during the early stages of aggregation and how the coexistence of native and misfolded conformers can significantly impact the aggregation process.

Characterizing protein oligomerization dynamics with automated mass photometry

Protein oligomerization is often difficult to be measured as some oligomeric species are only present at very low concentrations. Mass photometry is especially suited for assessing protein oligomerization, since it can quantify the relative abundance of a protein’s oligomeric states, including rare species. In this webinar, Svea Cheeseman explains how mass photometry and automated mass photometry work and shows data on two studies where the automated mass photometer, TwoMP Auto, was used to evaluate the oligomeric status of proteins of interest.

Harnessing mass photometry for AAV sample characterization in GMP-regulated environments

In this webinar, Cátia Crespo from Refeyn, explains how mass photometry measurement of AAV samples can be now applied to GMP-regulated environments. She discusses how Refeyn’s new software solutions, address all the US & EU regulatory requirements, including user management, audit trails and signing options when exporting files from Refeyn software applications. Additionally, she talks about how the new software solutions will be compatible with the SamuxMP and SamuxMP Auto mass photometers, which are optimized for AAV analytics, with the ability to accurately measure AAV empty/full ratios for any AAV serotype – using minimal sample volume.

Monitoring aggregation levels of biosimilar mAbs using mass photometry and SEC

In this webinar, Gareth Rogers from Refeyn, explains how mass photometry compares with size-exclusion chromatography in measuring aggregation levels of monoclonal antibodies such as trastuzumab, and several trastuzumab biosimilars. He discusses the pros and cons of each technology and summarizes some of the factors that should be considered when analyzing and comparing data generated by these different techniques.

Studying protein polymerization with mass photometry

In this webinar, Dr. Nikolas Hundt, a researcher at Ludwig Maximilian University of Munich, describes how he used mass photometry to investigate actin filament formation – by quickly quantifying all the species present in solution throughout the polymerization reaction. Mass photometry led him to a new interpretation of the mechanism behind actin polymerization and helped him revise established kinetic models.

Elucidating the composition of CRISPR-Cas12f1 complexes using mass photometry

Miniature CRISPR-Cas ribonucleoprotein complexes are of huge interest for genome editing applications due to their simplified cellular delivery. In this webinar, Selgar Henkel-Heinecke from Leipzig University describes how he and his colleagues used mass photometry to measure the stoichiometry of two miniature Cas12f1 ribonucleoprotein complexes, AsCas12f1 and SpCas12f1 in vitro, as well as their interaction with nucleic acids that are involved in the genome editing process.

Investigating membrane protein complexes with mass photometry

The speakers will present work studying two examples of macromolecular complexes formed by eukaryotic membrane proteins. In both cases, mass photometry was crucial to addressing highly relevant physiological questions. They will explain what mass photometry can do, and what makes it suitable for studies of membrane protein complexes.

Probing & modulating the function of the retromer endosomal trafficking complex through the novel macrocyclic peptides

In this webinar, find an explanation on how the macrocyclic peptides can be used as a novel toolbox to study retromer-mediated endosomal trafficking and for therapeutic targeting of retromer function. Highlighted, is the method mass photometry, which enables characterization of protein complexes.

Automated Mass Photometry : Easing the Path to Biomolecular Characterisation

In this webinar, we discuss in detail, automated mass photometry and show data on some of its most attractive applications—such as screening and titration assays. We illustrate how automation and the associated improvement in reproducibility can make biomolecular characterization with mass photometry even easier (TwoMP Auto Launch Webinar)

Probing Antibody Binding to the SARS-CoV-2 Spike Protein

Characterizing the mass of proteins and their complexes has a wide range of applications. However, the large degree of heterogeneity present in some biological systems has historically posed a near-unsurmountable challenge to traditional native mass spectrometry and classic structural biology techniques. To overcome this, researchers are turning to new, single-particle mass analysis methods. In this webinar, Victor Yin, a postdoctoral researcher of biomolecular mass spectrometry and proteomics at Utrecht University, discusses how mass photometry and charge detection mass spectrometry enable the study of several challenging protein systems, including the interaction of full antibodies with SARS-CoV-2.

Using Mass Photometry to Quantitate CaMKII stoichiometries

Prof. Margaret Stratton (UMass Amherst) presents her work on the role of CaMKII​ in different tissues. Mass photometry helps to quantitatively determine the stoichiometry of different variants of this crucial oligomeric enzyme.

Measuring the heterogeneity of macromolecular machines by mass photometry

In this webinar, Dr Adar Sonn-Segev presents how mass photometry provides quantitative information on sample heterogeneity using minimal volumes with molecular resolution. She also discusses how this approach applies to several different workflows including chemical crosslinking and multi-step purification, comparing it to the standard tools for measuring sample purity.

Applications of Mass Photometry

In this webinar, Refeyn co-founder Prof. Justin Benesch discusses examples of mass photometry measurements for large protein complexes and draws comparisons with mass measurements obtained from native mass spectrometry and other techniques. Dr Nikolas Hundt also describes the use of mass photometry to visualise the nucleation process of actin filaments in real time.

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