Mass photometry is a revolutionary way to measure the mass of molecules in their native states. Early mass photometry experiments, developed and pioneered by Philipp Kukura from the University of Oxford, imaged molecules on solid glass surfaces. However, many macromolecules, such as membrane-associated proteins and integral membrane proteins, perform their most important functions and interact with reaction partners in lipid membranes.
In this webinar, Nikolas Hundt, a former postdoctoral researcher in the Kukura laboratory, now at Ludwigs-Maximilian-University Munich, describes a new mass photometry strategy for unlabelled molecules diffusing on supported lipid bilayers. With this approach, called mass-sensitive particle tracking (MSPT), researchers can determine the mass distributions and diffusion characteristics of membrane-associated protein complexes and observe protein assembly dynamics on a lipid interface in real-time.
Nikolas Hundt is joined by Tamara Heermann and Frederik Steiert from the laboratory of Petra Schwille at the Max Planck Institute of Biochemistry during the question-and-answer session.