A mass photometer for AAV analytics
The Refeyn SamuxMP is a mass photometer optimized for adeno-associated virus (AAV) characterization and is an essential analytical tool for laboratories working with AAVs. SamuxMP mass photometry measurements are quick and require very little sample.
The SamuxMP precisely measures the empty/full capsid ratio for AAVs of any serotype as well as the quantities of partially filled and overfilled capsids. It can also detect impurities and aggregation, and give a rough capsid titer estimate as part of the standard data analysis procedure.
Quantitative characterisation of AAVs
-
Empty/full AAV ratio measurement
-
Serotype-independent analysis
-
Partially filled and overfilled capsid quantification
-
Rough estimation of titer
Rapid analysis requiring little sample
-
< 5 minutes to result
-
Little sample needed
-
Minimal sample preparation
-
Very low operating costs
User friendly operation
-
User training < 1 day
-
Software customised for AAV analysis
-
Benchtop instrument, Easy decontamination
-
GMP compliance coming soon
Transformative AAV analytics
MP
The Samux
Mass photometry is a bioanalytical technology that measures the mass of individual AAV particles in solution, quickly and using small amounts of sample.
Mass photometry is ideally suited for measurements of empty/full capsid ratios. Filled AAV capsids have greater mass than empty capsids, so they can be readily distinguished from empty capsids on mass photometry histograms (Fig 1).
Figure 1 Mass photometry measurement of AAV capsids (AAVDJ serotype) with SamuxMP. Two distinct peaks are visible: one with lower mass corresponding to empty capsids, and a second with higher mass corresponding to full capsids.
Figure 1 Mass photometry measurement of AAV capsids (AAVDJ serotype) with SamuxMP. Two distinct peaks are visible: one with lower mass corresponding to empty capsids, and a second with higher mass corresponding to full capsids.
Figure 2 Mass photometry measurements of empty AAV capsids of different serotypes on the SamuxMP. Mass histograms for four different AAV serotypes, with the mass and standard deviation calculated from three technical replicates.
To learn more about the SamuxMP and
mass photometry on AAVs, download our product sheet
Serotype-agnostic AAV analytics
The SamuxMP mass photometer can measure AAV capsids of any serotype without any adjustments.
Figure 2 shows SamuxMP measurements of empty AAV capsids of four different AAV serotypes.
For each sample, the mass photometry measurements with the SamuxMP revealed a single, symmetric peak at the expected mass, confirming that the SamuxMP produces consistent results across serotypes.
Further resources
Webinar: Are Your AAVs Filled? Determine Rapidly with Mass Photometry
In this webinar, you will learn more about the SamuxMP, our mass photometer tailored to address the challenges of AAV characterization. Moreover, you will see data proving its ability to reliably quantify the empty-full particle ratio for different AAV serotypes and at different purification levels. Watch it now on Refeyn’s YouTube channel!
Live demo : Watch the SamuxMP in action
Register for an upcoming remote demo on the SamuxMP, where a mass photometry expert will explain the principle behind the technology and demonstrate measurements of AAV samples on the instrument. There will also be an opportunity to ask questions in the Q&A session. Sign up to save your spot!
