Mass photometry analysis of protein-protein binding | Refeyn
Updated: May 16
We recently hosted a webinar featuring Dr. Grzegorz Piszczec, the Director of the Biophysics Core Facility at NIH, and Fabian Soltermann, a PhD candidate from the Kukura Group at the University of Oxford.
The webinar, now available on YouTube, discussed the following topics:
How mass photometry can accurately measure abundances of biomolecules in-vitro at the single-molecule level.
How we can use mass photometry to characterise and determine purity, stoichiometry, binding affinity and kinetics of multi-protein interactions.
How mass photometry results compare with those obtained by other biophysical methods.
More about a practical application of mass photometry: Quantifying interactions of antibody with Fc-receptors revealed influence of glycosylation and pH dependent interactions.
Which strategies can be used to obtain association equilibrium constant values from mass photometry data.
How to avoid pitfalls and overcome the limitations of mass photometry binding affinity measurements.
Associated publication:
Quantifying protein-protein interactions by molecular counting with mass photometry
Soltermann F, Foley E, Pagnoni V, et al. Angew. Chem. Int. Ed. 2020, 59,1–6. https://doi.org/10.1002/anie.202001578
You can watch the webinar on our YouTube channel - don't forget to subscribe!