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Quantifying protein binding affinities using mass photometry
Application Note

Protein interactions play pivotal roles in a wide range of biological processes. However, they are often highly complex, involving the binding of multivalent ligands to multiple binding sites. As a result, it can be challenging to identify the various complexes formed and assess the strength of their interactions.

Mass photometry is a fast, label-free technology that measures the molecular mass of individual biomolecules in solution, and is ideal for studying protein-protein interactions. By providing the mass distribution of biomolecules in a sample, it gives a detailed overview of the binding partners present, the complexes they form, the relative abundance of each species and the strength of their interactions.

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More Application Notes

Browse through our catalogue of application notes highlighting some recent case studies featuring mass photometry.

Mass photometry of nucleic acids

Mass photometry of nanocages

Mass photometry on SARS-CoV-2

Mass photometry on AAVs

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